Sunday, March 4, 2012, 9:00 am - 4:00 pm (with one hour break for lunch-on-your-own).  Tuition is $150.  

Instructors and Topic Areas:  

• Lisa Renee Olano, Ph.D., Postdoctoral Fellow, Bethesda, MD (Bioinformatics; specialist on label free and SILAC quantification) 
• Leesa Deterding, Ph.D., Staff Scientist, Research Triangle Park, NC (Quantitative proteomics of patient samples)
• Olga Vitek, Ph.D., Assistant Professor, Department of Computer Science, Purdue University (Methods for effective interpretation of spectral data for quanitative proteomics, metabolomics and ionomics)
• Sanford Markey, Ph.D., Bethesda, MD (Proteomic analysis in neurochemistry)

Course Description
Modern mass spectrometers provide a variety of platforms appropriate for the quantitative analyses of peptides from proteins.  This 6-hour course will approach the resulting options broadly, covering topics from MS1 ion current quantification to the myriad of acronyms (ITRAQ, MS(E), SILAC, MRM, SISCAPA).  We will present and discuss the many technical issues associated with quantitative analysis resulting from sample collection and preparation, protein digestion, and peptide chromatography; standards selection and use; MS1 data analysis (chromatographic run alignment, normalization); MS2 analysis (peak assignments, transition monitoring); statistical analyses of results; and study design.

Level:  Intermediate (Intended for those doing or planning quantitative proteomics; familiarity with mass spectrometry assumed)  

Go back to Short Course overview page.