There will be vendor lunch seminars scheduled on Monday and Tuesday. If your company is interested in hosting a vendor lunch seminar, please visit our exhibitor page
for order form.
Monday, March 20, 2017, 12:00 pm - 1:30 pm
Bruker - Grant Hall AB
Trapped ion mobility mass spectrometry (TIMS-MS) for shotgun proteomics, Scarlet Beck, Max Planck Institute of Biochemistry, Martinsried (near Munich), Germany
Ion mobility spectrometry (IMS) in combination with mass spectrometry (MS) has shown promise to increase the analysis speed, selectivity and sensitivity of MS experiments. Recently, we have introduced the Parallel Accumulation – Serial Fragmentation (PASEF) method on a prototype high-resolution trapped ion mobility spectrometry (TIMS)-MS instrument (Meier et al., JPR 2015). In PASEF mode, several precursors per ion mobility scan are selected, resulting in an increase in sequencing speed up to 10-fold. In this seminar we present the potential of TIMS-MS in combination with the PASEF method for shotgun proteomics applications.
Proteomic Profiling for Biomarker Discovery and Validation using a Bruker Impact II Q-TOF, Gary H. Kruppa, Ph. D., Vice President ‘Omics, Bruker Daltonics Inc., Billerica, MA
Proteoform profiling is showing great promise as a more efficient tool for biomarker discovery than bottom –up proteomics. Recent advances in proteoform profiling have been enabled by advances in both protein chromatography, and high resolution mass spectrometers. In this talk we will present HPLC-MS workflows using the Bruker Impact II Q-TOF mass spectrometer that result in the detection and label free quantitative comparison of >10,000 proteoforms per sample, in clinical research cohorts.
Register now for the Bruker seminar
Thermo Fisher Scientific - Grant Hall CD
The Essential Roles of System Qualifications, Inter-Sample QC and Intra-Sample QC in ‘omic Proteomics, M. Arthur Moseley, Ph.D. Associate Research Professor, Department of Medicine, Duke University School of Medicine
The continued evolution of mass spectrometry hardware and software has created myriad systems capable of highly reproducible analyses of complex mixtures (thousands of proteins) across large cohorts, including the native proteome and PTM-enriched proteomes (phosphoproteome, acetylome, ubiquitinome, etc.). However, without a strict set of analytical controls, one cannot routinely obtain the precision necessary for impactful biomedical projects. More importantly, our datasets must include samples that provide the hard metrics that accurately assess the reproducibility of the analysis, providing definitive metrics that allow statisticians to accurately differentiate between analytical variability and biological variability. This presentation will address the types of system qualification samples, QC samples and surrogate standards we have implemented into our protocols to accurately assess the reproducibility of our analyses, both proteomic and metabolomic, for use in clinical research projects.
Register onsite at the Thermo Fisher Scientific Booth #1. Space is limited.
Tuesday, March 21, 2017, 12:00 pm - 1:30 pm
SCIEX - Grant Hall AB
Enabling Precision Medicine with Industrialized Quantitative Proteomics, Randy Arnold & Christie Hunter, SCIEX
Many challenges exist in realizing the potential of precision medicine, especially in producing robust, reproducible quantitative proteomics measurements. This seminar will address many of the challenges that currently exist in making these measurements in a high-throughput, industrialized fashion:
• Automation and protein digestion kits for sample preparation reproducibility
• Microflow LC for enhanced workflow robustness and sample throughput
• Variable-window SWATH® Acquisition for comprehensive proteome coverage
• Cloud-based data processing to address the challenges of large data file and large sample numbers
• Integration with other omics data to provide more complete biological insight
Register now for the SCIEX seminar
Waters - Grant Hall CD
Quantitative Bottom up Proteomics by Data Independent Analysis using Quadrupole Scanning and Ion mobility, LeRoy B. Martin, III, Ph.D, Sr. Manager, Biological Mass Spectrometry, Waters Corporation
To quantitatively and qualitatively assess complex proteomic samples DIA analysis has been gaining in popularity. While the techniques have been shown to provide excellent, reproducible and comprehensive quantitative measurements, true qualitative assignments have always required additional specificity and peak capacity to generate the quality of data acceptable for database searching, library matching, and sequencing. In this workshop we'll present a novel quadrupole-scanning technique that maintains high dynamic range quantitative measurements while providing the selectivity necessary for proper qualitative characterization.
Register now for the Waters seminar